mouse anti human sox10 ab  (Proteintech)

Journal: PLoS ONE

Article Title: Contribution of Toll-Like Receptor 2 to the Innate Response against Staphylococcus aureus Infection in Mice

doi: 10.1371/journal.pone.0074287

Figure Lengend Snippet: To identify the infiltrating cell types and CD36 expression in the livers of infected WT mice and TLR2-deficient mice, murine liver tissues were immunostained for neutrophils, macrophages, and CD36, and analyzed for the CD36 gene expression level by real-time PCR. Representative photomicrographs (original magnification, × 200) of liver sections stained with an anti-MOP antibody for neutrophils at 6 h post-infection (A); an F4/80 antibody for macrophages at 2 d post-infection (B); and an anti-CD36 antibody for CD36 protein expression at 2 d post-infection (C); and the percentage of positively stained cells and the percentage of positively stained field areas are shown. Uninfected WT mice and TLR2-deficient mice served as controls. (D) Results from real-time PCR were normalized to GAPDH gene expression and are shown as fold changes relative to gene expression in the control mice. Data are presented as the mean ± SD for 5–7 mice per infected group and for 3 mice per uninfected group. * p < 0.05, # p < 0.01, $ p < 0.001 vs. uninfected WT mice; ∮ p < 0.05, ∞ p < 0.01, § p < 0.001 vs. uninfected TLR2-deficient mice.

Article Snippet: To detect F4/80 and CD36, 2-µm-thick tissue sections were immunostained with rabbit anti-mouse F4/80 Ab (Acris Antibodies Inc., CA, USA) and rabbit anti-human CD36 Ab (LifeSpan Biosciences Inc., WA, USA), respectively, followed by detection using the STAT-Q rapid IHC system (Innovex Biosciences Inc., CA, USA).

Techniques: Expressing, Infection, Real-time Polymerase Chain Reaction, Staining